Carestream Health Molecular Imaging Webinar Series
 |
These recorded presentations describe research technologies and techniques that utilize optical molecular imaging. Watch them any time when it’s convenient for you to learn how you can improve and extend your research.
To review the complete abstract for any of our webinars below, click on the title link. You will also be able to access the Webinar from the abstract page after entering basic contact information.
|
Upcoming Webinars
Check back soon for future webinars
Recorded Webinars
Jump to:
Multimodal Imaging of Drug Delivery
Examples of how imaging can aid in the rapid assessment of targeting strategies by allowing researchers to quickly study the in vivo biodistribution of labeled compounds, nanoparticles, peptides, and micelles will be illustrated.
Beyond Tumor, Rubor, Calor and Dolor: Using Molecular Imaging to Study Inflammation
In this webinar we will highlight examples of using molecular imaging to study and treat inflammatory diseases. For researchers interested in designing their own probes for inflammatory process, this seminar will highlight areas of much needed additional research as well as present tools for rapidly and accurately evaluating new probes and biomarkers for studying inflammatory processes.
Use of Indocyanine Green (ICG) in Small Animal In Vivo Imaging
ICG is an FDA approved NIR fluorescent dye, and is commercially available from a variety of companies. In this webinar we intend to highlight the use of ICG in whole animal in vivo imaging; its application and its limitations. In particular, we intend to highlight its use for imaging sites of inflammation through increased vascular leakage. We will also highlight peer reviewed work around utilizing this molecule for imaging increased inflammation at surgical sites.
Using Seven Imaging Modalities for Efficient and Accurate Evaluation of New Probes and Biomarkers In Small Animal Models
In the continued effort to better diagnose disease and predict therapeutic outcomes, researchers around the world have made significant efforts to validate new biomarkers and develop probes to image these in patients. Furthermore, to better understand the biological basis of disease in vivo, other researchers have been building new probes and biomarkers to report on biological processes at the cellular and tissue level strictly for pre-clinical research. While both of these areas have seen rapid progression over the past decade, there is still significant progress that can be made.
Utilizing the Multispectral Features of a Carestream Molecular Imaging In Vivo Multispectral System
Many in vivo fluorescent imaging experiments could be significantly enhanced through the application of multispectral technology. Multispectral technology allows for advanced multiplexing wherein fluorophores with closely overlapping spectrum can be neatly separated. This capability can be leverage to conduct multiple simultaneous molecular detections in a single in vivo sample. This capability can also be leveraged to remove autofluorescence which can be significant with in vivo fluorescent imaging. This session is intended to be instructional and will focus on the specifics of multispectral experimental design and workflow.
Non invasive imaging of plant physiology using optical and X-ray Imaging
The study of plant phenotypes, genotypes, and functional genomics is critical for understanding our environment and developing new solutions for food production. While new technology creates a deluge of sequence data, understanding the effect of mutations on phenotype can be an invasive and laborious process. Non-invasive imaging offers a solution overcoming this research bottle neck. Fluorescence and X-ray imaging, offer powerful tools for non-invasive imaging of biochemical pathways and plant physiology. This webinar will cover examples of imaging chlorophyll content, vasculature and host parasite interactions.
Imaging Nanoparticles and Validation of Specificity in In-Vivo Animal Models
New nanoparticles and nanotechnology designed for medical applications require rigorous evaluation both in vitro and in vivo. The purpose of many of these nanoparticles is to improve the treatment of disease through improved specificity, delivery of novel therapeutics, improved delivery of existing therapeutics, or delivery of novel combinations of therapeutics. In other cases, the goal of the nanoparticle may be improved detection or stratification of a disease. In all cases, in vivo imaging provides nanoparticle researchers a route to quickly validate the delivery, specificity, and efficacy of their novel therapeutics and imaging biomarkers in pre-clinical animal models. This presentation will review how multimodal molecular imaging can rapidly provide researchers a clear understanding of the delivery and efficacy of novel nanoparticles in pre-clinical animal models.
X-Ray Imaging Of Vascular Density In Mice
The goal of angiography is to study and visualize structural abnormalities in the vascular system. In traditional angiography, a radio opaque contrast is injected intravascularily and X-rays are used to visualize the vasculature. The most common radio opaque contrasts used in radiology are Iodine and Barium sulfate. Barium sulfate is highly cost effective and is routinely used to study gastro intestinal tract abnormalities.In this webinar, we will discuss the barium contrast angiogram technique using an X-ray source to acquire the high resolution images of the vasculature, which would be otherwise be obtained by expensive CT angiograms. We investigated the vascular heterogeneity in various vascular beds in different transgenic mice. This technique can be applied to visualize gross structural abnormalities in major vessels, such as aortic dissection, as well as in cancer studies. The use of barium sulfate with an X-ray system in this technique is highly cost effective and can be readily used in post-mortem angiography experiments.
Opportunities and Challenges of Imaging Antibodies In Vivo using Pre-clinical Small Animal Models
Researchers routinely use antibodies to visualize protein expression in ex vivo and in vitro samples. Western blots and immunohistochemistry are the top two applications for utilizing these approaches. Antibodies are relatively amenable to chemical conjugation of fluorophores and radiotracers, and can be purchased as pre-labeled compounds. Given the widespread availability of high quality antibodies, why haven’t they been used for in vivo research in a systematic way? In this webinar, we intended to highlight the challenges posed by utilizing antibodies in vivo, but also highlight key applications where antibodies can successfully be used in small animal models.
Non-Invasive In Vivo Imaging of Cell Death via Apoptosis or Necrosis
How do cells decide whether to live or die? Answering this seemingly simple question has engaged researchers across a variety of disciplines including neuroscience, pharmacology, oncology, cardiology, and development. Much of this work answering these questions has been necessarily studied in model organisms and in cultured cells. Yet the most critical questions involving cell fate often require the necessarily complex environment of a living animal such as a mouse or rat. Paracrine and autocrine signals as well as relative immunological response and 3D structure often play a decisive role in the fate of cells in live animals, and many of these conditions are difficult to replicate in vitro.
Multimodal Optical and Nuclear Imaging
Preclinical applications of biomedical imaging are rapidly expanding as tools for molecular biology research and diagnostic contrast agent development. As no single imaging modality can provide a full picture of the complex biology of disease, more researchers are turning to multimodality imaging. The availability of contrast agents for optical and nuclear imaging has never been higher, including both genetically engineered reporters and synthetic compounds. In this webinar, we will discuss the principles of planar optical imaging and scintigraphy in the context of imaging in animal models. These techniques are economical and simple, allowing facile data co-registration. We will review multimodal imaging strategies with respect to laboratory research and clinical applications.
Optical Imaging of Anionic Membranes for Detecting and Monitoring Disease States In-Vivo
In this webinar, we will discuss the development of a series of fluorescent probes, each composed of synthetic zinc (II)-dipicolylamine affinity ligand conjugated to a near-infrared dye. These small molecule probes can target anionic membranes, permitting detection of bacterial infection, tumors, and tissue damage in living animals.
Introduction to Optical Imaging for Investigating Cancer Biology and Relevant Pathways
Recently, near-infrared and red fluorescent probes have enabled significant advances in understanding the role of proteins and enzymes in tumor biology in-vivo.
Taking Your Images from Grayscale to Great
Fluorescent multiplex Western blotting has become a widely-used method for fast, quantitative protein expression analyses in which the sample and control proteins of interest can be simultaneously incubated then sequentially imaged to produce a composite overlay. Multiplex Western analysis involves incubation of two or more primary antibodies from different hosts followed by incubation with two or more spectrally distinct fluorescent secondary antibodies. The variety of excitation and emission filter combinations available on the Carestream Image Station 4000MM PRO, IS4000MM PRO with Fixed Lens and the Gel Logic 6000 PRO (430 to 830nm) allows multiplex Western detection of two or more proteins using most commercially available conjugated fluorophores, such as CyDyes™, Alexa Fluors®, Qdots®, IRDyes®, and DyLights™. Once the experiment is optimized, images are captured using appropriate filter combinations for each fluorophore. Individual images can then be pseudocolored, contrasted, and finally overlayed to produce publication-quality figures. Carestream Molecular Imaging (MI) Software has two possible ways to complete this task; a simple layered 2-color overlay using the Image Display menu or blending up to four images using the Multiplex Analysis window. Regardless of the method used, the final overlayed image can be easily exported using a variety of image file formats for incorporation into papers and/or grants.
Illumination Sources for Fluorescence Applications
Fluorescent molecules are beacons that can absorb photons of light of a higher energy (short wavelength) and then emit photons at a longer wavelength with less energy. These fluorescent molecules have been demonstrated to be useful for a wide variety of applications in biological research. For example, in-vitro assays such as microscopy and cell sorting (FACS) have used both single as well as multiplex labeling approaches to address complex questions. More recently, fluorescent molecules have been gaining utility as labels for antibodies to generate probes for detection of proteins in Western blotting applications.
Whatever the application, fluorescent molecules require a source of excitation. This can be delivered by three main types of illumination sources: lasers, broad spectrum filtered light and, more recently, light emitting diodes (LED’s). This webinar will present some of the pros and cons for each type of illumination source with an emphasis on their use for detection of labels in fluorescence Western blotting applications.
Simultaneous Chemiluminescent and Fluorescent Detection
The ability to multiplex fluorescent labels has greatly improved the utility of applications like Western blotting. The simultaneous detection of 2 or more protein targets provides both cost and time-savings increasing lab productivity. While fluorescence imaging is rapidly advancing, chemiluminescent Western blot analysis still remains the most sensitive method for detection of low-copy protein targets. Although most loading control proteins are highly expressed proteins that can be easily detected using fluorescent labels, most targets of interest are low-copy number proteins that demand the greatest potential detection sensitivity. To address this need we have developed a method that accommodates simultaneous incubation of both fluorescent and chemiluminescent labeled secondary antibodies. This webinar will outline this approach and present data to support the validity and reliability of the method. Signal output enhancers and post image processing for presentation and analysis will also be discussed.
Using TEM Film Today
In this webinar, we will review when it is advantageous to use film in particular imaging situations. Discussions will also include using film in conjunction with digital imaging to provide optimum image quality results, while maintaining a productive work flow
Advancing In Vitro Molecular Imaging: Introducing the Gel Logic 6000 PRO
This presentation will focus on an overview of this new system’s capabilities and expanded features
Practical Techniques for Image Optimization Using the Multimodal Animal Rotation System (MARS) with the MS FX PRO Multispectral Imaging System
This webinar is designed to demonstrate some useful methods for optimizing MARS applications on various areas of the body using the MS FX PRO multispectral imaging system and therefore providing enhanced image quality and improved statistical analysis.
Expanding Digital Imaging Capabilities: Introducing the Gel Logic 4000 PRO
This presentation will focus on an overview of the Gel Logic 4000 PRO system’s capabilities and expanded features.
Applications and Experiment Flexibility with the Carestream Image Station 4000MM PRO
New and innovative features of the Image Station 4000MM PRO and highlight how these new features can improve the workflow of molecular imaging, as well as provide applications and experiment flexibility with Carestream’s most advanced in vitro imaging system.
Introducing the Albira System - A Revolutionary PET/SPECT/CT Combination
The Albira system combines PET, SPECT, and CT imaging in six novel and extremely powerful ways. The system’s highly compact, multi-modal modular design gives you the freedom to purchase what you need now, and upgrade as you research needs evolve.
An Introduction to the Multimodal Animal Rotation System (MARS)
Carestream Molecular Imaging developed the Multimodal Animal Rotation System (MARS) to enable researchers to automatically acquire both incremental and arbitrary series of multimodal images of a mouse from multiple angles, as much as a seamless 360° scan, in one capture session.
Utilizing A High Resolution X-Ray System For Basic And Pre-Clinical Research
In this webinar, we shall demonstrate how quantitative, small animal X-ray systems provide an ideal solution for non-invasive imaging a both the skeleton or soft tissue structures in pre-clinical animal models.
Introducing Carestream Molecular Imaging’s New Bone Density Software
Carestream is pleased to announce the release of our newest accessory for our advanced MI Software. With this powerful new addition, you can now perform bone density and bone mass analysis of small animals in vivo and mineral density of in-vitro specimens.
In Vitro Digital Imaging – An Applications Overview
This webinar will review Carestream’s Image Station line and will highlight a variety of applications (i.e., single and multi-wavelength fluorescence, luminescence, colorimetric, and radioisotopic assays) that can be performed.
Fully Automated Digital Imaging: Introducing the Gel Logic 2200PRO
The Gel Logic 2200 PRO is the new automated workhorse of the Gel Logic imagers. Aimed at the entry level luminescent imaging market, the GL 2200 PRO offers integrated trans-UV and trans-white light illumination for versatile imaging of protein and DNA based gels.
The Next Evolution in Gel Documentation Systems: Introducing the Gel Logic 212 PROThis webinar will introduce Carestream Molecular Imaging's next-generation gel documentation system, the new, fully automated Gel Logic 212 PRO.
Taking Your First Step into Digital Imaging: The Gel Logic 112
The Carestream Gel Logic 112 is designed for research labs who want a simple, inexpensive solution to everyday imaging needs. The 112 is a high quality, cost effective documentation device that allows you to easily and quickly image gels (both protein and nucleic acid), blots, plates, assays, and other in vitro specimens.
Tips and Techniques
Defining Regions of Interest (ROIs) on Gels and Western Blots
This presentation will discuss these approaches and their strengths and weaknesses for analysis of both gels and Western blots.
Tips and Techniques: Optimization of Gel Logic Imaging Parameters
This webinar will provide basic parameters for imaging some of these different types of samples as well as tips for optimizing capture of these images.
Tips and Techniques: The In-Vivo MS FX PRO for the Beginning User
This session will provide attendees with an orientation to the In-Vivo MS FX PRO system and capabilities.
Getting the Most from Your Display, Analysis, and Reporting Features using Carestream/Kodak Molecular Imaging Software
This presentation will review the wide range of image display, analysis, and reporting tools that uniquely complements the capture output of molecular imaging systems.